comparison of matrix metalloproteinases 2 mrna expression in prostatic adenocarcinoma and benign prostatic hyperplasia

نویسندگان

bahareh rahmatizadeh

farzaneh tafvizi

masoud salehipoure

چکیده

background and aims: prostate cancer is the second most common cancer in men worldwide in men. matrix metalloproteinase 2 (mmp2) has a role in the invasion and destruction of the basement membrane and the extra-cellular matrix and facilitating the process of tumor cell invasion. the present study was conducted to compare the expression of mmp2 gene in prostate cancer (pca) and benign prostatic hyperplasia (bph). materials and methods: 48 samples of pca and 48 samples of bph (as controls) were examined. rna was extracted from paraffin tissue blocks and then used for cdna synthesize. the real time polymerase chain reaction method was used to quantify the expression of mmp2 gene. the level of gene expression was assessed using participants’ clinicopathological characteristics. results: the expression of mmp2 gene increased in the pca samples compared with the bph samples (p=0.003). mmp2 gene increased in all the grades of this group compared with the controls. the highest expression, however, was observed in the moderately differentiated grade. no significant correlations or relationships were observed in the pca samples between prostate specific antigen and the other study variables such as age, mmp2 expression, disease grade and the gleason score; however a positive correlation was observed between prostate specific antigen and the samples' pathological stage. conclusions: the expression of mmp2 increased in the pca samples compared with the bph samples, with the highest expression occurring in the samples with a gleason score of 6. further tests and studies conducted with larger sample sizes may help to use this marker in differentiating malignant from benign samples.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of Matrix Metalloproteinases 2 mRNA Expression in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia

Background and Aims: Prostate cancer is the second most common cancer in men worldwide in men. Matrix metalloproteinase 2 (MMP2) has a role in the invasion and destruction of the basement membrane and the extra-cellular matrix and facilitating the process of tumor cell invasion. The present study was conducted to compare the expression of MMP2 gene in prostate cancer (PCa) and benign prostatic ...

متن کامل

Evaluation of c-Myc mRNA Expression Level in Benign Prostatic Hyperplasia and Prostatic Adenocarcinoma Tissues and Its Correlation with Clinicopathological Characteristics

Background and Aims: Prostate cancer (PCa) is one of the most common cancers among men in Iran. Since changes in the regulation of proto-oncogenes expression are the main causes of most human cancers, including PCa, evaluating the expression of marker genes can be helpful for early diagnosis of cancer and better understanding of its etiology. The present study compared c-Myc expression level in...

متن کامل

Non – Detection of HPV DNA in Prostatic Cancer and Benign Prostatic Hyperplasia: a case- control study in Kerman

Background: Different studies assessed the role of inflammation in malignancy of different organs. Prostatitis has been suggested as a cause of cancer and BPH. Till now, more than 100 types of human Papilloma virus (HPV) are recognized including low and high-risk groups for carcinogenesis. Among them HPV-16 and HPV-18 have shown further association with cancer. Regarding the pr...

متن کامل

Benign prostatic hyperplasia.

This article reviews the epidemiology of benign prostatic hyperplasia (BPH), evaluation of patients with lower urinary tract symptomatology (LUTS), and management of patients with BPH and LUTS. The evaluation includes history and physical examination, laboratory testing, and specialized studies. The management includes medical and surgical options. Medical options include alpha-1-adrenergic rec...

متن کامل

Benign Prostatic Hyperplasia

Urinary obstruction as a result of benign prostatic disease has probably been recognized to some degree since the earl iest days of medicine. This association was probably f irst formalized by Riolan in the seventeenth century. In the mideighteenth century, Morgagni (248) provided one of the earl iest descriptions of BPH and enumerated many of the potential medical problems attendant to its dev...

متن کامل

Benign Prostatic Hyperplasia (bph)

Comprehensive studies show that the holmium laser is ideal for treating BPH. Holmium laser treatments have been the most rigorously investigated BPH techniques. Over 90 articles have been published from 8 randomized clinical trials and over 30 case studies and comparative series. The American, European, British and Australasian urology associations recommend holmium laser for BPH treatment. Hol...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
مجله بین المللی علوم آزمایشگاهی

جلد ۳، شماره ۴، صفحات ۲۲۹-۲۴۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023